PI3 Kinase p85 Beta Polyclonal antibody

PI3 Kinase p85 Beta Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25868-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, U-937 cells
Positive IHC detected inhuman placenta tissue, mouse kidney tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25868-1-AP targets PI3 Kinase p85 Beta in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 PI3 Kinase p85 Beta fusion protein Ag14369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 2 (beta)
别名 p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2
计算分子量 728 aa, 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC014170
基因名称 PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
RRIDAB_3669503
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p85 Beta antibody 25868-1-APDownload protocol
IHC protocol for PI3 Kinase p85 Beta antibody 25868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nanomaterials (Basel)

2D MXene Nanosheets with ROS Scavenging Ability Effectively Delay Osteoarthritis Progression

Authors - Hongqi Zhao
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