验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, U-937 cells |
| Positive IHC detected in | human placenta tissue, mouse kidney tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25868-1-AP targets PI3 Kinase p85 Beta in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Immunogen | PI3 Kinase p85 Beta fusion protein Ag14369 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphoinositide-3-kinase, regulatory subunit 2 (beta) |
| Synonyms | p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2 |
| Calculated Molecular Weight | 728 aa, 82 kDa |
| Observed Molecular Weight | 82 kDa |
| GenBank Accession Number | BC014170 |
| Gene Symbol | PI3 Kinase p85 Beta |
| Gene ID (NCBI) | 5296 |
| RRID | AB_3669503 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p85 Beta antibody 25868-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p85 Beta antibody 25868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
