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PIAS1 Monoclonal antibody

PIAS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

10A12E6

Cat no : 60498-1-Ig

Print datasheet

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

验证数据展示

  • WB analysis using 60498-1-Ig

    WB analysis using 60498-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 60498-1-Ig

    WB analysis of THP-1 using 60498-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMOLT-4 cells, THP-1 cells, Jurkat cells, IMR-32 cells
Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60498-1-Ig targets PIAS1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PIAS1 fusion protein Ag18132 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein inhibitor of activated STAT, 1
Synonyms EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIAS1 antibody 60498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols