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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 60498-1-Ig

    WB analysis using 60498-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 60498-1-Ig

    WB analysis of THP-1 using 60498-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PIAS1 Monoclonal antibody

PIAS1 Monoclonal Antibody for WB, ELISA
Cat No. 60498-1-Ig
CloneNo. 10A12E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMOLT-4 cells, THP-1 cells, Jurkat cells, IMR-32 cells
Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60498-1-Ig targets PIAS1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PIAS1 fusion protein Ag18132 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein inhibitor of activated STAT, 1
Synonyms EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
RRIDAB_3670265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIAS1 antibody 60498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols