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PIAS2 Polyclonal antibody

PIAS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16074-1-AP

Print datasheet

Synonyms

E3 SUMO-protein transferase PIAS2, E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3

验证数据展示

  • WB analysis using 16074-1-AP

    WB analysis using 16074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 16074-1-AP

    IF Staining of MCF-7 using 16074-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 16074-1-AP (PIAS2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16074-1-AP targets PIAS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen PIAS2 fusion protein Ag9032 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein inhibitor of activated STAT, 2
Synonyms E3 SUMO-protein transferase PIAS2, E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3
Calculated Molecular Weight 63 kDa, 69 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015190
Gene Symbol PIAS2
Gene ID (NCBI) 9063
RRIDAB_2164208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for PIAS2 antibody 16074-1-APDownload protocol
IF protocol for PIAS2 antibody 16074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article
humanWB

Biochim Biophys Acta Mol Cell Res

IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5

Authors - Junfeng Du
View Article
humanWB,IF

J Mol Med (Berl)

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Authors - Yuhong Zhang
View Article