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PIAS2 Recombinant antibody, PBS Only (Capture)

PIAS2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241474G8

Cat no : 84189-4-PBS

Print datasheet

Synonyms

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

验证数据展示

  • WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

    WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

    WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-1-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-2-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84189-4-RR

    Affinity and Kinetic Characterization of 84189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

产品信息

84189-4-PBS targets PIAS2 as part of a matched antibody pair:

MP01062-1: 84189-4-PBS capture and 84189-1-PBS detection (validated in Cytometric bead array)

MP01062-2: 84189-4-PBS capture and 84189-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIAS2 fusion protein Ag9032 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein inhibitor of activated STAT, 2
Synonyms E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3
Calculated Molecular Weight 63 kDa, 69 kDa
Observed Molecular Weight63-68 kDa
GenBank Accession NumberBC015190
Gene Symbol PIAS2
Gene ID (NCBI) 9063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75928
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.