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验证数据展示

  • WB analysis of HL-60 using 18469-1-AP

    WB analysis of HL-60 using 18469-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18469-1-AP

    IF Staining of HeLa using 18469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIAS4 antibody (18469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 18469-1-AP

    FC experiment of HeLa using 18469-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PIAS4 (18469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PIAS4 Polyclonal antibody

PIAS4 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 18469-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18469-1-AP targets PIAS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PIAS4 fusion protein Ag13316 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein inhibitor of activated STAT, 4
别名 PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27
计算分子量 57 kDa
观测分子量 57-70 kDa
GenBank蛋白编号BC010047
基因名称 PIAS4
Gene ID (NCBI) 51588
RRIDAB_2164380
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N2W9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

实验方案

Product Specific Protocols
WB protocol for PIAS4 antibody 18469-1-APDownload protocol
IF protocol for PIAS4 antibody 18469-1-APDownload protocol
FC protocol for PIAS4 antibody 18469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols