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  • KD/KO Validated

PIBF1 Polyclonal antibody

PIBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14413-1-AP

Print datasheet

Synonyms

Progesterone-induced-blocking factor 1, Progesterone induced blocking factor 1, PIBF 1, Centrosomal protein of 90 kDa



经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, K-562 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14413-1-AP targets PIBF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human
Cited Reactivityhuman
Immunogen PIBF1 fusion protein Ag5755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progesterone immunomodulatory binding factor 1
Synonyms Progesterone-induced-blocking factor 1, Progesterone induced blocking factor 1, PIBF 1, Centrosomal protein of 90 kDa
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC051911
Gene Symbol PIBF1
Gene ID (NCBI) 10464
RRIDAB_10858916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXW3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

实验方案

Product Specific Protocols
WB protocol for PIBF1 antibody 14413-1-APDownload protocol
IHC protocol for PIBF1 antibody 14413-1-APDownload protocol
IF protocol for PIBF1 antibody 14413-1-APDownload protocol
IP protocol for PIBF1 antibody 14413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP, IF

Elife

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Authors - Andrew Kodani
  • KD Validated
humanWB

J Cell Biol

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Authors - Dhivya Kumar
  • KO Validated
humanIF

EMBO Rep

Zika virus alters centrosome organization to suppress the innate immune response.

Authors - Andrew Kodani
humanIF

PLoS Biol

The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly

Authors - Pierrick Le Borgne
  • KD Validated
humanIF

Cell Rep

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Authors - H Kubra Gurkaslar
humanIF

EMBO J

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis

Authors - Marine H Laporte