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PICALM Polyclonal antibody

PICALM Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 28554-1-AP

Print datasheet

Synonyms

CALM, CLTH, LAP, PICALM

验证数据展示

  • WB analysis using 28554-1-AP

    WB analysis using 28554-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28554-1-AP

    IHC staining of mouse brain using 28554-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28554-1-AP targets PICALM in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB,ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PICALM fusion protein Ag29505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol binding clathrin assembly protein
Synonyms CALM, CLTH, LAP, PICALM
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC064357
Gene Symbol PICALM
Gene ID (NCBI) 8301
RRIDAB_2918175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

实验方案

Product Specific Protocols
WB protocol for PICALM antibody 28554-1-APDownload protocol
IHC protocol for PICALM antibody 28554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols