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PICALM Monoclonal antibody

PICALM Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6G7

Cat no : 67564-1-Ig

Print datasheet

Synonyms

CALM, CLTH, LAP, PICALM

验证数据展示

  • WB analysis using 67564-1-Ig

    WB analysis using 67564-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67564-1-Ig

    IHC staining of human lung cancer using 67564-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67564-1-Ig

    IF Staining of HeLa using 67564-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67564-1-Ig targets PICALM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PICALM fusion protein Ag29512 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylinositol binding clathrin assembly protein
Synonyms CALM, CLTH, LAP, PICALM
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC064357
Gene Symbol PICALM
Gene ID (NCBI) 8301
RRIDAB_2882778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

实验方案

Product Specific Protocols
WB protocol for PICALM antibody 67564-1-IgDownload protocol
IHC protocol for PICALM antibody 67564-1-IgDownload protocol
IF protocol for PICALM antibody 67564-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols