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0
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验证数据展示

  • WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

    IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

    IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

    IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

    IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

    Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PICK1 Polyclonal antibody, PBS Only

PICK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10983-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10983-2-PBS targets PICK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PICK1 fusion protein Ag1443 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein interacting with PRKCA 1
别名
计算分子量 47 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC017561
基因名称 PICK1
Gene ID (NCBI) 9463
RRIDAB_2164525
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRD5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.