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  • Featured Product
  • KD/KO Validated

PICK1 Polyclonal antibody, PBS Only

PICK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10983-2-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

    IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

    IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

    IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

    IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

    Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

产品信息

10983-2-PBS targets PICK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PICK1 fusion protein Ag1443 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein interacting with PRKCA 1
Synonyms
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC017561
Gene Symbol PICK1
Gene ID (NCBI) 9463
RRIDAB_2164525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRD5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.