验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
19006-1-AP targets PIF1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PIF1 fusion protein Ag13522 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) |
| 别名 | PIF1/RRM3 DNA helicase-like protein, PIF, EC:3.6.4.12, DNA repair and recombination helicase PIF1, C15orf20 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 63-73 kDa |
| GenBank蛋白编号 | BC018978 |
| 基因名称 | PIF1 |
| Gene ID (NCBI) | 80119 |
| RRID | AB_10598312 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H611 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIF1, also named as ATP-dependent DNA helicase PIF1, is a 641 amino acid protein, which belongs to the helicase family. PIF1 as a DNA-dependent ATPase and 5'-3' DNA helicase is required for the maintenance of both mitochondrial and nuclear genome stability. PIF1 localizes in the nucleus and is involved in the maintenance of telomeric DNA. PIF1 exists as four isoforms. isoform 1 is a 70 kDa protein and isoform 2 is 63 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIF1 antibody 19006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
