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验证数据展示

  • WB analysis of HeLa using 13679-1-AP

    WB analysis of HeLa using 13679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 13679-1-AP

    WB analysis of HEK-293 using 13679-1-AP

    WB result of PIGA antibody (13679-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIGA transfected HEK-293 cells.

  • IHC staining of mouse lung using 13679-1-AP

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13679-1-AP

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

PIGA Polyclonal antibody

PIGA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13679-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Synonyms

GlcNAc PI synthesis protein, GPI3, PIG A, PIGA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13679-1-AP targets PIGA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PIGA fusion protein Ag4530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class A
Synonyms GlcNAc PI synthesis protein, GPI3, PIG A, PIGA
Calculated Molecular Weight 484 aa, 54 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC038236
Gene Symbol PIGA
Gene ID (NCBI) 5277
RRIDAB_2164656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP37287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGA antibody 13679-1-APDownload protocol
IHC protocol for PIGA antibody 13679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF,WB

Hum Mol Genet

CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model.

Authors - Marshall Lukacs
  • KO Validated
View Article