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PIGB Polyclonal antibody

PIGB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12612-1-AP

Print datasheet

Synonyms

GPI mannosyltransferase 3, GPI mannosyltransferase III, GPI MT III, PIG B, PIGB

验证数据展示

  • WB analysis of human liver using 12612-1-AP

    WB analysis of human liver using 12612-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12612-1-AP (PIGB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12612-1-AP

    IHC staining of human prostate cancer using 12612-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12612-1-AP (PIGB antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman liver tissue, human brain tissue, mouse testis tissue, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12612-1-AP targets PIGB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PIGB fusion protein Ag3244 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class B
Synonyms GPI mannosyltransferase 3, GPI mannosyltransferase III, GPI MT III, PIG B, PIGB
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC017711
Gene Symbol PIGB
Gene ID (NCBI) 9488
RRIDAB_2164664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92521
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGB antibody 12612-1-APDownload protocol
IHC protocol for PIGB antibody 12612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem J

Accelerated urokinase-receptor protein turnover triggered by interference with the addition of the glycolipid anchor.

Authors - Avila Hector H
View Article