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PIGK Polyclonal antibody

PIGK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15151-1-AP

Print datasheet

Synonyms

GPI anchor transamidase, GPI transamidase, GPI8, GPI8 homolog, hGPI8, PIG K, PIGK

验证数据展示

  • WB analysis of HT-1080 using 15151-1-AP

    WB analysis of HT-1080 using 15151-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15151-1-AP

    IHC staining of human ovary tumor using 15151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 15151-1-AP

    IHC staining of human ovary tumor using 15151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHT-1080 cells, SMMC-7721 cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15151-1-AP targets PIGK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen PIGK fusion protein Ag3497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Synonyms GPI anchor transamidase, GPI transamidase, GPI8, GPI8 homolog, hGPI8, PIG K, PIGK
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC026186
Gene Symbol PIGK
Gene ID (NCBI) 10026
RRIDAB_2164682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGK antibody 15151-1-APDownload protocol
IHC protocol for PIGK antibody 15151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
View Article
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
View Article