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验证数据展示

  • WB analysis of HeLa using 16369-1-AP

    WB analysis of HeLa using 16369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16369-1-AP

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16369-1-AP

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 16369-1-AP

    IF Staining of MCF-7 using 16369-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PIGO Polyclonal antibody

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16369-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, mouse spleen tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16369-1-AP targets PIGO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PIGO fusion protein Ag9232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol glycan anchor biosynthesis, class O
别名 UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein
计算分子量1089aa,119 kDa; 454aa,50 kDa
观测分子量 74 kDa
GenBank蛋白编号BC013987
基因名称 PIGO
Gene ID (NCBI) 84720
RRIDAB_2164806
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGO antibody 16369-1-APDownload protocol
IHC protocol for PIGO antibody 16369-1-APDownload protocol
IF protocol for PIGO antibody 16369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols