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PIGO Polyclonal antibody

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16369-1-AP

Print datasheet

Synonyms

UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein

验证数据展示

  • WB analysis of HeLa using 16369-1-AP

    WB analysis of HeLa using 16369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16369-1-AP

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16369-1-AP

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 16369-1-AP

    IF Staining of MCF-7 using 16369-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, mouse spleen tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16369-1-AP targets PIGO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PIGO fusion protein Ag9232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class O
Synonyms UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein
Calculated Molecular Weight1089aa,119 kDa; 454aa,50 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC013987
Gene Symbol PIGO
Gene ID (NCBI) 84720
RRIDAB_2164806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGO antibody 16369-1-APDownload protocol
IHC protocol for PIGO antibody 16369-1-APDownload protocol
IF protocol for PIGO antibody 16369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols