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PIGT Polyclonal antibody
PIGT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, A549 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16906-1-AP targets PIGT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PIGT fusion protein Ag10521 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphatidylinositol glycan anchor biosynthesis, class T |
| Synonyms | UNQ716/PRO1379, PSEC0163, Phosphatidylinositol-glycan biosynthesis class T protein, NDAP, GPI transamidase component PIG-T |
| Calculated Molecular Weight | 578 aa, 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC015022 |
| Gene Symbol | PIGT |
| Gene ID (NCBI) | 51604 |
| RRID | AB_2164836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969N2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIGT is a subunit of the glycosylphosphatidylinositol transamidase complex that catalyzes the attachment of proteins to GPI-anchors. GPI-anchors are glycolipids found on membrane of diverse cells that help proteins anchoring to the cell surface. Mutations of PIGT have been linked to developmental disorders including multiple congenital anomalies-hypotonia-seizures syndrome-3. Multiple isoforms of PIGT exist due to the alternative splicing, with the predicted MWs ranging from 42 kDa to 66 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIGT antibody 16906-1-AP | Download protocol |
| IHC protocol for PIGT antibody 16906-1-AP | Download protocol |
| IF protocol for PIGT antibody 16906-1-AP | Download protocol |
| IP protocol for PIGT antibody 16906-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking
| ||
CNS Neurosci Ther Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury | ||
Nat Commun Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis | ||
BMC Med Genomics Proteomic analysis illustrates the potential involvement of dysregulated ribosome-related pathways and disrupted metabolism during retinoic acid-induced cleft palate development |
