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PIGZ Polyclonal antibody

PIGZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17384-1-AP

Print datasheet

Synonyms

GPI mannosyltransferase 4, GPI mannosyltransferase IV, GPI MT IV, hSMP3, PIG Z, PIGZ, SMP3, SMP3 homolog

验证数据展示

  • WB analysis of human brain using 17384-1-AP

    WB analysis of human brain using 17384-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17384-1-AP

    IHC staining of human colon using 17384-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HepG2 using 17384-1-AP

    IF Staining of HepG2 using 17384-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17384-1-AP (PIGZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, human testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17384-1-AP is PIGZ Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PIGZ fusion protein Ag11259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class Z
Synonyms GPI mannosyltransferase 4, GPI mannosyltransferase IV, GPI MT IV, hSMP3, PIG Z, PIGZ, SMP3, SMP3 homolog
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC044640
Gene Symbol PIGZ
Gene ID (NCBI) 80235
RRIDAB_10697833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGZ antibody 17384-1-APDownload protocol
IHC protocol for PIGZ antibody 17384-1-APDownload protocol
IF protocol for PIGZ antibody 17384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Phenomics

A Genome-Wide Association Study for Susceptibility to Axial Length in Highly Myopic Eyes

Authors - Qiang Lu
View Article
humanWB

J Biol Chem

Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis

Authors - JingFeng Li
View Article