PIH1D1 Polyclonal antibody
PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human testis tissue, human uterus tissue, mouse eye tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19427-1-AP targets PIH1D1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PIH1D1 fusion protein Ag13759 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PIH1 domain containing 1 |
| Synonyms | PIH1 domain-containing protein 1, PIH1 domain containing 1, Nucleolar protein 17 homolog, NOP17 |
| Calculated Molecular Weight | 290 aa, 32 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC001108 |
| Gene Symbol | PIH1D1 |
| Gene ID (NCBI) | 55011 |
| RRID | AB_10640423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIH1D1 antibody 19427-1-AP | Download protocol |
| IHC protocol for PIH1D1 antibody 19427-1-AP | Download protocol |
| IF protocol for PIH1D1 antibody 19427-1-AP | Download protocol |
| IP protocol for PIH1D1 antibody 19427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium. | ||
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
Cell Chem Biol RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. | ||
Chromosoma The PRDM9 KRAB domain is required for meiosis and involved in protein interactions. | ||
J Biol Chem Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex. |
