PIH1D1 Polyclonal antibody

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19427-1-AP

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Synonyms

PIH1 domain-containing protein 1, PIH1 domain containing 1, Nucleolar protein 17 homolog, NOP17



经过测试的应用

Positive WB detected inA431 cells, human testis tissue, human uterus tissue, mouse eye tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19427-1-AP targets PIH1D1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PIH1D1 fusion protein Ag13759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PIH1 domain containing 1
Synonyms PIH1 domain-containing protein 1, PIH1 domain containing 1, Nucleolar protein 17 homolog, NOP17
Calculated Molecular Weight 290 aa, 32 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC001108
Gene Symbol PIH1D1
Gene ID (NCBI) 55011
RRIDAB_10640423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

实验方案

Product Specific Protocols
WB protocol for PIH1D1 antibody 19427-1-APDownload protocol
IHC protocol for PIH1D1 antibody 19427-1-APDownload protocol
IF protocol for PIH1D1 antibody 19427-1-APDownload protocol
IP protocol for PIH1D1 antibody 19427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Nat Commun

The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.

Authors - Chloé Maurizy
ratWB

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
human

RNA

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

Authors - Machado-Pinilla Rosario R
humanWB

Cell Chem Biol

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.

Authors - Paul Yenerall
mouseWB

Chromosoma

The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.

Authors - Yukiko Imai
mouseWB

J Biol Chem

Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.

Authors - Janakiram R Vangala