验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, human placenta tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse ovary tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PIK3C2A Ready-To-Use IHC Kit. PIK3C2A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | PIK3C2A fusion protein Ag16894 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, class 2, alpha polypeptide |
| 别名 | CPK, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A |
| 计算分子量 | 1686 aa, 191 kDa |
| 观测分子量 | 191 kDa |
| GenBank蛋白编号 | BC113658 |
| 基因名称 | PIK3C2A |
| Gene ID (NCBI) | 5286 |
| RRID | AB_11183760 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00443 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| IHC protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| IF protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| IP protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.
| ||
Cancer Cell Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function. | ||
Cell Res Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation. | ||
J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization
| ||
J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization. | ||
Front Cell Infect Microbiol The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection. |
