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PIK3C2B Polyclonal antibody

PIK3C2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24788-1-AP

Print datasheet

Synonyms

C2 PI3K, PI3K C2 beta, PIK3C2B

验证数据展示

  • WB analysis using 24788-1-AP

    WB analysis using 24788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24788-1-AP (PIK3C2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24788-1-AP

    IP experiment of HeLa using 24788-1-AP

    IP result of anti-PIK3C2B (IP:24788-1-AP, 4ug; Detection:24788-1-AP 1:1500) with HeLa cells lysate 1240 ug.

  • IHC staining of human skeletal muscle using 24788-1-AP

    IHC staining of human skeletal muscle using 24788-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 24788-1-AP

    IHC staining of human skeletal muscle using 24788-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 24788-1-AP

    IF Staining of HeLa using 24788-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24788-1-AP (PIK3C2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24788-1-AP is PIK3C2B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PIK3C2B fusion protein Ag20474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, class 2, beta polypeptide
Synonyms C2 PI3K, PI3K C2 beta, PIK3C2B
Calculated Molecular Weight 1634 aa, 185 kDa
Observed Molecular Weight 160-185 kDa
GenBank Accession NumberBC132876
Gene Symbol PIK3C2B
Gene ID (NCBI) 5287
RRIDAB_2879725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00750
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIK3C2B antibody 24788-1-APDownload protocol
IHC protocol for PIK3C2B antibody 24788-1-APDownload protocol
IF protocol for PIK3C2B antibody 24788-1-APDownload protocol
IP protocol for PIK3C2B antibody 24788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Anal Chem

Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes.

Authors - Jiawen Lyu
View Article
humanWB

Cell Rep

ALKBH5 causes retinal pigment epithelium anomalies and choroidal neovascularization in age-related macular degeneration via the AKT/mTOR pathway

Authors - Ru-Xu Sun
View Article