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PI3 Kinase p110 Alpha Polyclonal antibody

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21890-1-AP

Print datasheet

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

验证数据展示

  • IP experiment of mouse heart using 21890-1-AP

    IP experiment of mouse heart using 21890-1-AP

    IP result of anti-PI3 Kinase p110 Alpha (IP:21890-1-AP, 4ug; Detection:21890-1-AP 1:300) with mouse heart tissue lysate 3600 ug.

  • IF Staining of MCF-7 using 21890-1-AP

    IF Staining of MCF-7 using 21890-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human brain using 21890-1-AP

    WB analysis of human brain using 21890-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21890-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21890-1-AP

    IHC staining of human lung cancer using 21890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIK3CA antibody included
  • IHC staining of human lung cancer using 21890-1-AP

    IHC staining of human lung cancer using 21890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21890-1-AP (PI3K p110(alpha) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIK3CA antibody included

经过测试的应用

Positive WB detected inhuman brain tissue, Jurkat cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PIK3CA Ready-To-Use IHC Kit. PIK3CA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21890-1-AP is PI3 Kinase p110 Alpha Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PI3 Kinase p110 Alpha fusion protein Ag16586 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Synonyms p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha
Calculated Molecular Weight 1068 aa, 124 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 21890-1-APDownload protocol
IHC protocol for PI3 Kinase p110 Alpha antibody 21890-1-APDownload protocol
IF protocol for PI3 Kinase p110 Alpha antibody 21890-1-APDownload protocol
IP protocol for PI3 Kinase p110 Alpha antibody 21890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
View Article
humanWB

J Agric Food Chem

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.

Authors - Heshan Xu
View Article
humanWB

J Hematol Oncol

Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression.

Authors - Yang Li
View Article
mouseWB,IHC

Sci Rep

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Authors - Shu Yang
View Article
humanWB

J Exp Clin Cancer Res

LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.

Authors - Bing Liu
View Article
mouseWB

Sci Rep

Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines.

Authors - Koichi Baba
View Article