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验证数据展示

  • WB analysis of LNCaP using 67964-1-Ig

    WB analysis of LNCaP using 67964-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67964-1-Ig

    WB analysis of Jurkat using 67964-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 67964-1-Ig

    IF Staining of LNCaP using 67964-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody (67964-1-Ig, Clone: 2G11C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PI3 Kinase p110 Delta Monoclonal antibody

PI3 Kinase p110 Delta Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67964-1-Ig

产品说明书

CloneNo. 2G11C6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF/ICC detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PI3 Kinase p110 Delta fusion protein Ag26995 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, delta polypeptide
别名 p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta
计算分子量 1044 aa, 120 kDa
观测分子量110 kDa
GenBank蛋白编号BC132919
基因名称 PIK3CD
Gene ID (NCBI) 5293
RRIDAB_2918715
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
IF protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols