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PIK3CG Polyclonal antibody

PIK3CG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31229-1-AP

Print datasheet

Synonyms

Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, p120-PI3K, p120 PI3K, p110gamma, EC:2.7.11.1

验证数据展示

  • WB analysis using 31229-1-AP

    WB analysis using 31229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31229-1-AP (PIK3CG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31229-1-AP

    IHC staining of human ovary cancer using 31229-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31229-1-AP (PIK3CG antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31229-1-AP targets PIK3CG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIK3CG fusion protein Ag34951 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, catalytic, gamma polypeptide
Synonyms Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, p120-PI3K, p120 PI3K, p110gamma, EC:2.7.11.1
Calculated Molecular Weight 126 kDa
Observed Molecular Weight126 kDa
GenBank Accession NumberBC035683
Gene Symbol PIK3CG
Gene ID (NCBI) 5294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP48736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIK3CG antibody 31229-1-APDownload protocol
IHC protocol for PIK3CG antibody 31229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols