验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16826-1-AP targets PIK3IP1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, bovine |
| 免疫原 | PIK3IP1 fusion protein Ag10427 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase interacting protein 1 |
| 别名 | HGFL, hHGFL(S), PIK3IP1 |
| 计算分子量 | 263 aa, 28 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC011049 |
| 基因名称 | PIK3IP1 |
| Gene ID (NCBI) | 113791 |
| RRID | AB_2163333 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96FE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3IP1(Phosphoinositide-3-kinase-interacting protein 1) is also named as HGFL. The class IA phosphoinositol-3-kinases (PI3Ks) regulate important cellular processes such as proliferation, growth, survival, motility and metabolism. PIK3IP1 is a transmembrane protein that possesses a region in its intracellular domain that shares homology with the p85 regulatory subunit of PI3K. It has 5 isoforms produced by alternative splicing with the MW of 11, 18, 19, 25,28 kDa. PIK3IP1 undergoes N- and O-linked amino acid glycosylation. The major glycosylated form of PIK3IP1 migrates at about 43 kDa in western blot analysis, while the unglycosylated form migrates at 37 kDa. It is also detected a 65 kDa variant in HepG2 cell lysate that PIK3IP1 is indeed membrane bound and that these variants arise from alternative splicing and/or post-translational processing events such as enzymatic proteolysis and/or glycosylation. (PMID:18632611).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIK3IP1 antibody 16826-1-AP | Download protocol |
| IP protocol for PIK3IP1 antibody 16826-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism. | ||
Nat Commun Dual blockade of the lipid kinase PIP4Ks and mitotic pathways leads to cancer-selective lethality.
| ||
Oncogene PPM1D activity promotes cellular transformation by preventing senescence and cell death | ||
J Immunol Cutting Edge: Foxp1 Controls Naive CD8+ T Cell Quiescence by Simultaneously Repressing Key Pathways in Cellular Metabolism and Cell Cycle Progression. | ||
Sci Rep Downregulation of PIK3IP1 in retinal microglia promotes retinal pathological neovascularization via PI3K-AKT pathway activation |
