- Featured Product
- KD/KO Validated
PI3 Kinase p55 Gamma Polyclonal antibody
PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HUVEC cells, HEK-293 cells |
Positive IHC detected in | human kidney tissue, mouse testis tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
产品信息
27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | PI3 Kinase p55 Gamma fusion protein Ag25884 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phosphoinositide-3-kinase, regulatory subunit 3 (gamma) |
Synonyms | p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3 |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC021622 |
Gene Symbol | PI3 Kinase p55 Gamma |
Gene ID (NCBI) | 8503 |
RRID | AB_2880730 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92569 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PI3 Kinase p55 Gamma antibody 27035-1-AP | Download protocol |
IHC protocol for PI3 Kinase p55 Gamma antibody 27035-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Physiol Lung Cell Mol Physiol p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model. | ||
BMC Cancer Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma
| ||
World J Mens Health Relaxin-2 Prevents Erectile Dysfunction by Cavernous Nerve, Endothelial and Histopathological Protection Effects in Rats with Bilateral Cavernous Nerve Injury | ||