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  • Featured Product
  • KD/KO Validated

PI3 Kinase p55 Gamma Polyclonal antibody

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27035-1-AP

Print datasheet

Synonyms

p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3

验证数据展示

  • WB analysis of MCF-7 using 27035-1-AP

    WB analysis of MCF-7 using 27035-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

  • WB analysis of HEK-293 using 27035-1-AP

    WB analysis of HEK-293 using 27035-1-AP

    WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells.

  • WB analysis of HUVEC using 27035-1-AP

    WB analysis of HUVEC using 27035-1-AP

    WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College).

  • IHC staining of human kidney using 27035-1-AP

    IHC staining of human kidney using 27035-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27035-1-AP

    IHC staining of human kidney using 27035-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse testis using 27035-1-AP

    IHC staining of mouse testis using 27035-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 27035-1-AP

    IHC staining of mouse kidney using 27035-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen PI3 Kinase p55 Gamma fusion protein Ag25884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Synonyms p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC021622
Gene Symbol PI3 Kinase p55 Gamma
Gene ID (NCBI) 8503
RRIDAB_2880730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
IHC protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Physiol Lung Cell Mol Physiol

p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model.

Authors - Ting Wang
View Article
human,mouseWB,IHC

BMC Cancer

Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma

Authors - Cheng Tan
  • KD Validated
View Article
ratWB

World J Mens Health

Relaxin-2 Prevents Erectile Dysfunction by Cavernous Nerve, Endothelial and Histopathological Protection Effects in Rats with Bilateral Cavernous Nerve Injury

Authors - Kang Liu
View Article