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验证数据展示

  • WB analysis using 81857-1-RR

    WB analysis using 81857-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81857-1-RR (PIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 81857-1-RR

    WB analysis of HeLa using 81857-1-RR

    WB result of PIN1 antibody (81857-1-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells.

  • IP experiment of HepG2 using 81857-1-RR

    IP experiment of HepG2 using 81857-1-RR

    IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with HepG2 cells lysate 1920 ug.

  • IF Staining of NIH/3T3 using 81857-1-RR

    IF Staining of NIH/3T3 using 81857-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (81857-1-RR, Clone: 5N20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of NIH/3T3 using 81857-1-RR

    IP experiment of NIH/3T3 using 81857-1-RR

    IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with NIH/3T3 cells lysate 1120 ug.

  • IHC staining of human pancreas cancer using 81857-1-RR

    IHC staining of human pancreas cancer using 81857-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIN1 antibody included
  • IHC staining of human pancreas cancer using 81857-1-RR

    IHC staining of human pancreas cancer using 81857-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIN1 antibody included
  • Affinity and Kinetic Characterization of 81857-1-RR

    Affinity and Kinetic Characterization of 81857-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81857-1-RR against Human PIN1 were performed. The affinity constant is 0.17 nM.

  • Featured Product
  • KD/KO验证

PIN1 Recombinant antibody

PIN1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 81857-1-RR

产品说明书

CloneNo. 5N20

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8, 5N20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IP detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81857-1-RR targets PIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PIN1 fusion protein Ag0767 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidylprolyl cis/trans isomerase, NIMA-interacting 1
别名 Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8, 5N20
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC002899
基因名称 PIN1
Gene ID (NCBI) 5300
RRIDAB_2935587
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

实验方案

Product Specific Protocols
WB protocol for PIN1 antibody 81857-1-RRDownload protocol
IHC protocol for PIN1 antibody 81857-1-RRDownload protocol
IF protocol for PIN1 antibody 81857-1-RRDownload protocol
IP protocol for PIN1 antibody 81857-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols