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  • Featured Product
  • KD/KO Validated

PIN4 Polyclonal antibody

PIN4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15789-1-AP

Print datasheet

Synonyms

Peptidyl-prolyl cis/trans isomerase EPVH, Parvulin-17, Parvulin-14, hPar14, EPVH

验证数据展示

  • IHC staining of human liver cancer using 15789-1-AP

    IHC staining of human liver cancer using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIN4 antibody included
  • IHC staining of human liver cancer using 15789-1-AP

    IHC staining of human liver cancer using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIN4 antibody included
  • WB analysis of mouse liver using 15789-1-AP

    WB analysis of mouse liver using 15789-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15789-1-AP

    IF Staining of HepG2 using 15789-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIN4 antibody (15789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PIN4 Ready-To-Use IHC Kit. PIN4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15789-1-AP targets PIN4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PIN4 fusion protein Ag8489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
Synonyms Peptidyl-prolyl cis/trans isomerase EPVH, Parvulin-17, Parvulin-14, hPar14, EPVH
Calculated Molecular Weight 131 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC005234
Gene Symbol PIN4
Gene ID (NCBI) 5303
RRIDAB_2878186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIN4, also known as EPVH, hPar14, is a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. PIN4 is the first eukaryotic member of the parvulin family of PPIases and contains a PPIase domain preceded by a 40-amino acid glycine- and lysine-rich N-terminal sequence.

实验方案

Product Specific Protocols
WB protocol for PIN4 antibody 15789-1-APDownload protocol
IHC protocol for PIN4 antibody 15789-1-APDownload protocol
IF protocol for PIN4 antibody 15789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation

Authors - Miki Naito
  • KD Validated
View Article