验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A431 cells, C6 cells, NIH/3T3 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 8 publications below |
产品信息
12368-1-AP targets PINX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PINX1 fusion protein Ag3043 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PIN2-interacting protein 1 |
| 别名 | Pin2-interacting protein X1, Pin2 interacting protein X1, PIN2 interacting protein 1, LPTS, LPTL |
| 计算分子量 | 328 aa, 37 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC015479 |
| 基因名称 | PINX1 |
| Gene ID (NCBI) | 54984 |
| RRID | AB_2164405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BK5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PINX1 antibody 12368-1-AP | Download protocol |
| IF protocol for PINX1 antibody 12368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.
| ||
Mol Cancer The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway. | ||
Am J Cancer Res PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.
| ||
Mol Cancer PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway. | ||
Cancer Sci Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma. |
