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  • Featured Product
  • KD/KO Validated

PIP4K2A Polyclonal antibody

PIP4K2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12469-1-AP

Print datasheet

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

验证数据展示

  • WB analysis of mouse brain using 12469-1-AP

    WB analysis of mouse brain using 12469-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12469-1-AP

    IP experiment of mouse brain using 12469-1-AP

    IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • IHC staining of human spleen using 12469-1-AP

    IHC staining of human spleen using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PIP4K2A antibody included
  • IHC staining of human spleen using 12469-1-AP

    IHC staining of human spleen using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PIP4K2A antibody included
  • IF Staining of HepG2 using 12469-1-AP

    IF Staining of HepG2 using 12469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black).

  • WB analysis of mouse brain using 12469-1-AP

    WB analysis of mouse brain using 12469-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 12469-1-AP

    WB analysis using 12469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12469-1-AP

    WB analysis using 12469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 12469-1-AP

    IF Staining of SH-SY5Y using 12469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy PIP4K2A Ready-To-Use IHC Kit. PIP4K2A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12469-1-AP targets PIP4K2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PIP4K2A fusion protein Ag3152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Synonyms Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha
Calculated Molecular Weight 406 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC018034
Gene Symbol PIP4K2A
Gene ID (NCBI) 5305
RRIDAB_10639518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48426
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIP4K2A antibody 12469-1-APDownload protocol
IHC protocol for PIP4K2A antibody 12469-1-APDownload protocol
IF protocol for PIP4K2A antibody 12469-1-APDownload protocol
IP protocol for PIP4K2A antibody 12469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF,IHC

Dev Cell

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Authors - Archna Ravi
View Article
humanIF,IHC,IP,WB

Int J Biochem Cell Biol

A novel lncRNA lncSAMD11-1:1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization

Authors - Mei Zhang
View Article
human,mouseWB,IHC,IF

Sci Adv

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition

Authors - Joanna Triscott
  • KD Validated
View Article