验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PIP4K2B Ready-To-Use IHC Kit. PIP4K2B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
13218-1-AP targets PIP4K2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | PIP4K2B fusion protein Ag4099 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol-5-phosphate 4-kinase, type II, beta |
| 别名 | PI(5)P 4 kinase type II beta, PI5P4KB, PIP4K2B, PIP4KII beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta |
| 计算分子量 | 416 aa, 47 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC027459 |
| 基因名称 | PIP4K2B |
| Gene ID (NCBI) | 8396 |
| RRID | AB_10644482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78356 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. It binds TNFRSF1A(24/32/50kd).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIP4K2B antibody 13218-1-AP | Download protocol |
| IHC protocol for PIP4K2B antibody 13218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
