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PIP4K2B Recombinant antibody

PIP4K2B Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240645F10

Cat no : 83647-3-RR

Print datasheet

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

验证数据展示

  • WB analysis using 83647-3-RR

    WB analysis using 83647-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83647-3-RR

    IHC staining of mouse brain using 83647-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • IHC staining of mouse brain using 83647-3-RR

    IHC staining of mouse brain using 83647-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2B antibody included
  • FC experiment of A549 using 83647-3-RR

    FC experiment of A549 using 83647-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, A549 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PIP4K2B Ready-To-Use IHC Kit. PIP4K2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83647-3-RR targets PIP4K2B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIP4K2B fusion protein Ag4099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Synonyms Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10
Calculated Molecular Weight 416 aa, 47 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC027459
Gene Symbol PIP4K2B
Gene ID (NCBI) 8396
RRIDAB_3671256
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.

实验方案

Product Specific Protocols
WB protocol for PIP4K2B antibody 83647-3-RRDownload protocol
IHC protocol for PIP4K2B antibody 83647-3-RRDownload protocol
FC protocol for PIP4K2B antibody 83647-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols