验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, A549 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83647-3-RR targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | PIP4K2B fusion protein Ag4099 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | phosphatidylinositol-5-phosphate 4-kinase, type II, beta |
别名 | Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10 |
计算分子量 | 416 aa, 47 kDa |
观测分子量 | 45 kDa |
GenBank蛋白编号 | BC027459 |
基因名称 | PIP4K2B |
Gene ID (NCBI) | 8396 |
RRID | AB_3671256 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P78356 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.
实验方案
Product Specific Protocols | |
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WB protocol for PIP4K2B antibody 83647-3-RR | Download protocol |
IHC protocol for PIP4K2B antibody 83647-3-RR | Download protocol |
IF protocol for PIP4K2B antibody 83647-3-RR | Download protocol |
FC protocol for PIP4K2B antibody 83647-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |