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验证数据展示

  • WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

PIP5K1A Monoclonal antibody, PBS Only

PIP5K1A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67483-1-PBS

产品说明书

CloneNo. 3E5G12

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67483-1-PBS targets PIP5K1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 PIP5K1A fusion protein Ag8355 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
别名 PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha
计算分子量 56 kDa, 63 kDa
观测分子量 63 kDa
GenBank蛋白编号BC007833
基因名称 PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2882710
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99755
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.