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验证数据展示

  • WB analysis of HEK-293 using 17547-1-AP

    WB analysis of HEK-293 using 17547-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 17547-1-AP

    IF Staining of HEK-293 using 17547-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17547-1-AP (PIP5KL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PIP5KL1 Polyclonal antibody

PIP5KL1 Polyclonal Antibody for WB, IF, ELISA
Cat No. 17547-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17547-1-AP targets PIP5KL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PIP5KL1 fusion protein Ag11653 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase-like 1
别名 PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH
计算分子量 22 kDa, 44 kDa
观测分子量 45 kDa
GenBank蛋白编号BC042184
基因名称 PIP5KL1
Gene ID (NCBI) 138429
RRIDAB_2252440
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T9C9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIP5KL1 antibody 17547-1-APDownload protocol
IF protocol for PIP5KL1 antibody 17547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols