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PIPPIN Polyclonal antibody

PIPPIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25013-1-AP

Print datasheet

Synonyms

CSDC2, RNA-binding protein PIPPin, RNA binding protein PIPPin, Cold shock domain-containing protein C2

验证数据展示

  • WB analysis of mouse brain using 25013-1-AP

    WB analysis of mouse brain using 25013-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25013-1-AP (PIPPIN antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25013-1-AP

    IHC staining of human kidney using 25013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25013-1-AP targets PIPPIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIPPIN fusion protein Ag16216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cold shock domain containing C2, RNA binding
Synonyms CSDC2, RNA-binding protein PIPPin, RNA binding protein PIPPin, Cold shock domain-containing protein C2
Calculated Molecular Weight 153 aa, 17 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC067113
Gene Symbol CSDC2
Gene ID (NCBI) 27254
RRIDAB_2879845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y534
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).

实验方案

Product Specific Protocols
WB protocol for PIPPIN antibody 25013-1-APDownload protocol
IHC protocol for PIPPIN antibody 25013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols