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验证数据展示

  • WB analysis of HepG2 using 10263-1-AP

    WB analysis of HepG2 using 10263-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10263-1-AP

    IP experiment of HeLa using 10263-1-AP

    IP result of anti-PIR (IP:10263-1-AP, 4ug; Detection:10263-1-AP 1:300) with HeLa cells lysate 1200ug.

  • IHC staining of human stomach using 10263-1-AP

    IHC staining of human stomach using 10263-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10263-1-AP (PIR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PIR Polyclonal antibody

PIR Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10263-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Probable quercetinase, Probable quercetin 2,3-dioxygenase PIR, Pirin, EC:1.13.11.24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10263-1-AP targets PIR in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 PIR fusion protein Ag0330 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pirin (iron-binding nuclear protein)
别名 Probable quercetinase, Probable quercetin 2,3-dioxygenase PIR, Pirin, EC:1.13.11.24
计算分子量 32 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002517
基因名称 PIR
Gene ID (NCBI) 8544
RRIDAB_2283879
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00625
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

实验方案

Product Specific Protocols
WB protocol for PIR antibody 10263-1-APDownload protocol
IHC protocol for PIR antibody 10263-1-APDownload protocol
IP protocol for PIR antibody 10263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

PIR promotes tumorigenesis of breast cancer by upregulating cell cycle activator E2F1.

Authors - Muhammad Suleman
  • KD Validated
View Article