• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PIR Monoclonal antibody

PIR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10E4

Cat no : 67516-1-Ig

Print datasheet

Synonyms

PIR, Pirin

验证数据展示

  • WB analysis of HSC-T6 using 67516-1-Ig

    WB analysis of HSC-T6 using 67516-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHSC-T6 cells, HeLa cells, K-562 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67516-1-Ig targets PIR in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PIR fusion protein Ag29969 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pirin (iron-binding nuclear protein)
Synonyms PIR, Pirin
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002517
Gene Symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2882737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

实验方案

Product Specific Protocols
WB protocol for PIR antibody 67516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols