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PIRT Polyclonal antibody

PIRT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 20990-1-AP

Print datasheet

Synonyms

hCG_1776018, PIRT, TRPV1 complex

验证数据展示

  • WB analysis of SH-SY5Y using 20990-1-AP

    WB analysis of SH-SY5Y using 20990-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20990-1-AP (PIRT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20990-1-AP targets PIRT in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity human
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-interacting regulator of transient receptor potential channels
Synonyms hCG_1776018, PIRT, TRPV1 complex
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_001101387
Gene Symbol PIRT
Gene ID (NCBI) 644139
RRIDAB_2878785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C851
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIRT is a key component of the TRPV1 complex that positively regulates TRPV1 activity. It binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI). PIRT is a membrane protein with MW 15-25 kDa.

实验方案

Product Specific Protocols
WB protocol for PIRT antibody 20990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Evid Based Complement Alternat Med

C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4.

Authors - Xing-Qi Bao
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mouseIHC

EMBO Rep

Defective branched-chain amino acid catabolism in dorsal root ganglia contributes to mechanical pain

Authors - Huijing Xie
View Article