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PISD Monoclonal antibody

PISD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B6C1

Cat no : 68484-1-Ig

Print datasheet

Synonyms

DJ858B16, dJ858B16.2, DKFZp566G2246, PISD, PSDC, PSSC

验证数据展示

  • WB analysis using 68484-1-Ig

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68484-1-Ig

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68484-1-Ig targets PISD in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PISD fusion protein Ag9499 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylserine decarboxylase
Synonyms DJ858B16, dJ858B16.2, DKFZp566G2246, PISD, PSDC, PSSC
Calculated Molecular Weight375aa,43 kDa; 408aa,46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001482
Gene Symbol PISD
Gene ID (NCBI) 23761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UG56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PISD antibody 68484-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols