PITPNA Polyclonal antibody

PITPNA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16613-1-AP

Print datasheet

Synonyms

PI TP alpha, PITPN, PITPNA, PtdIns transfer protein alpha, PtdInsTP alpha, VIB1A



经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung tissue, human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PITPNA Ready-To-Use IHC Kit. PITPNA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16613-1-AP targets PITPNA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen PITPNA fusion protein Ag9849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol transfer protein, alpha
Synonyms PI TP alpha, PITPN, PITPNA, PtdIns transfer protein alpha, PtdInsTP alpha, VIB1A
Calculated Molecular Weight 270 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC045108
Gene Symbol PITPNA
Gene ID (NCBI) 5306
RRIDAB_2299692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PITPNA antibody 16613-1-APDownload protocol
IHC protocol for PITPNA antibody 16613-1-APDownload protocol
IP protocol for PITPNA antibody 16613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Dev Cell

A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis.

Authors - Zhigang Xie
ratWB

BMC Complement Altern Med

Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats.

Authors - Gao Yong-Hui YH
mouseWB,IF

Nat Commun

Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction

Authors - Yu-Te Yeh