验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung tissue, human brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PITPNA Ready-To-Use IHC Kit. PITPNA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
16613-1-AP targets PITPNA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | PITPNA fusion protein Ag9849 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol transfer protein, alpha |
| 别名 | PI TP alpha, PITPN, PITPNA, PtdIns transfer protein alpha, PtdInsTP alpha, VIB1A |
| 计算分子量 | 270 aa, 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC045108 |
| 基因名称 | PITPNA |
| Gene ID (NCBI) | 5306 |
| RRID | AB_2299692 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00169 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PITPNA antibody 16613-1-AP | Download protocol |
| IHC protocol for PITPNA antibody 16613-1-AP | Download protocol |
| IP protocol for PITPNA antibody 16613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. | ||
BMC Complement Altern Med Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats. | ||
Nat Commun Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction |
