验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, A549 cells, mouse testis tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human osteosarcoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
10101-2-AP targets PITRM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PITRM1 fusion protein Ag0150 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pitrilysin metallopeptidase 1 |
| 别名 | MP1, Metalloprotease 1, KIAA1104, hPreP, hMP1 |
| 计算分子量 | 60 kDa, 117 kDa |
| 观测分子量 | 110-117 kDa |
| GenBank蛋白编号 | BC001150 |
| 基因名称 | PITRM1 |
| Gene ID (NCBI) | 10531 |
| RRID | AB_2165145 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JRX3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PITRM1 antibody 10101-2-AP | Download protocol |
| IHC protocol for PITRM1 antibody 10101-2-AP | Download protocol |
| IF protocol for PITRM1 antibody 10101-2-AP | Download protocol |
| IP protocol for PITRM1 antibody 10101-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
