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验证数据展示

  • WB analysis using 30674-1-AP

    WB analysis using 30674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30674-1-AP (PITRM1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 30674-1-AP

    IHC staining of human breast cancer using 30674-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included
  • IHC staining of human breast cancer using 30674-1-AP

    IHC staining of human breast cancer using 30674-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included

PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30674-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PITRM1 Ready-To-Use IHC Kit. PITRM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30674-1-AP targets PITRM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 PITRM1 fusion protein Ag30478 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pitrilysin metallopeptidase 1
别名 hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP
计算分子量 60 kDa, 117 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC001150
基因名称 PITRM1
Gene ID (NCBI) 10531
RRIDAB_3086385
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JRX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.

实验方案

Product Specific Protocols
WB protocol for PITRM1 antibody 30674-1-APDownload protocol
IHC protocol for PITRM1 antibody 30674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols