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PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30674-1-AP

Print datasheet

Synonyms

hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP

验证数据展示

  • WB analysis using 30674-1-AP

    WB analysis using 30674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30674-1-AP (PITRM1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 30674-1-AP

    IHC staining of human breast cancer using 30674-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included
  • IHC staining of human breast cancer using 30674-1-AP

    IHC staining of human breast cancer using 30674-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PITRM1 antibody included

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PITRM1 Ready-To-Use IHC Kit. PITRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30674-1-AP targets PITRM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen PITRM1 fusion protein Ag30478 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pitrilysin metallopeptidase 1
Synonyms hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP
Calculated Molecular Weight 60 kDa, 117 kDa
Observed Molecular Weight110-120 kDa
GenBank Accession NumberBC001150
Gene Symbol PITRM1
Gene ID (NCBI) 10531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.

实验方案

Product Specific Protocols
WB protocol for PITRM1 antibody 30674-1-APDownload protocol
IHC protocol for PITRM1 antibody 30674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols