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PIWIL1 Polyclonal antibody, PBS Only

PIWIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15659-1-PBS

Print datasheet

Synonyms

Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI

验证数据展示

  • WB analysis of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    WB analysis of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    IHC staining of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15659-1-AP (same clone as 15659-1-PBS)

    IF Staining of HepG2 using 15659-1-AP (same clone as 15659-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    IP experiment of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:2000) with mouse testis tissue lysate 2400 ug. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    IF Staining of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PIWIL1 antibody (15659-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

产品信息

15659-1-PBS targets PIWIL1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PIWIL1 fusion protein Ag8056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name piwi-like 1 (Drosophila)
Synonyms Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC028581
Gene Symbol PIWIL1
Gene ID (NCBI) 9271
RRIDAB_2878163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96J94
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C