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PJA1 Polyclonal antibody
PJA1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17687-1-AP targets PJA1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PJA1 fusion protein Ag11820 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | praja ring finger 1 |
Synonyms | PJA1, praja ring finger 1, Praja1, RING finger protein 70, RNF70 |
Calculated Molecular Weight | 643 aa, 71 kDa |
Observed Molecular Weight | 71-80 kDa |
GenBank Accession Number | BC075803 |
Gene Symbol | PJA1 |
Gene ID (NCBI) | 64219 |
RRID | AB_2252483 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8NG27 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PJA1 antibody 17687-1-AP | Download protocol |
IP protocol for PJA1 antibody 17687-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.
| ||
Ann Clin Transl Neurol A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system. | ||
J Virol PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation. |