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PKC Gamma Monoclonal antibody, PBS Only

PKC Gamma Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4B9

Cat No : 66429-1-PBS

Print datasheet

Synonyms

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

验证数据展示

  • WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

产品信息

66429-1-PBS targets PKC Gamma in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PKC Gamma fusion protein Ag5910 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein kinase C, gamma
Synonyms PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC047876
Gene Symbol PRKCG
Gene ID (NCBI) 5582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05129
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.