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PKC Gamma Monoclonal antibody, PBS Only

PKC Gamma Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4B9

Cat no : 66429-1-PBS

Print datasheet

Synonyms

PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13

验证数据展示

  • WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    FC experiment of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (66429-1-Ig, Clone:2F4B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

产品信息

66429-1-PBS targets PKC Gamma in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity pig, rat, mouse, human
Immunogen PKC Gamma fusion protein Ag5910 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein kinase C, gamma
Synonyms PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC047876
Gene Symbol PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2881800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05129
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.