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  • Featured Product
  • KD/KO Validated

PKC Iota Monoclonal antibody, PBS Only

PKC Iota Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3A1B6

Cat No : 66493-1-PBS

Print datasheet

Synonyms

aPKC lambda/iota, DXS1179E, PKC Iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota

验证数据展示

  • WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCI antibody included
  • IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCI antibody included
  • IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKCI Ready-To-Use IHC Kit. PRKCI primary antibody included.

产品信息

66493-1-PBS targets PKC Iota in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, rat, mouse
Immunogen PKC Iota fusion protein Ag4990 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein kinase C, iota
Synonyms aPKC lambda/iota, DXS1179E, PKC Iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC022016
Gene Symbol PRKCI
Gene ID (NCBI) 5584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41743
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.