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验证数据展示

  • WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCI antibody included
  • IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCI antibody included
  • IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PKC Iota Monoclonal antibody, PBS Only

PKC Iota Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66493-1-PBS

产品说明书

CloneNo. 3A1B6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

PRKCI, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota, aPKC-lambda/iota

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKCI Ready-To-Use IHC Kit. PRKCI primary antibody included.

产品信息

66493-1-PBS targets PKC Iota in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PKC Iota fusion protein Ag4990 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase C, iota
别名 PRKCI, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota, aPKC-lambda/iota
计算分子量 68 kDa
观测分子量 70 kDa
GenBank蛋白编号BC022016
基因名称 PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2881858
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41743
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.