PKC Zeta Polyclonal antibody
PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HT-29 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26899-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | PKC Zeta fusion protein Ag25391 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein kinase C, zeta |
Synonyms | PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13 |
Calculated Molecular Weight | 80 kDa |
Observed Molecular Weight | 67 kDa |
GenBank Accession Number | BC014270 |
Gene Symbol | PKC Zeta |
Gene ID (NCBI) | 5590 |
RRID | AB_2880675 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q05513 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PKC Zeta antibody 26899-1-AP | Download protocol |
IHC protocol for PKC Zeta antibody 26899-1-AP | Download protocol |
IF protocol for PKC Zeta antibody 26899-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS Pathog HRS plays an important role for TLR7 signaling to orchestrate inflammation and innate immunity upon EV71 infection. | ||
JCI Insight Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy. | ||
Fish Shellfish Immunol In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I. | ||
J Cell Mol Med Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis. | ||
Cell Rep Med Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling
| ||
Dev Cell Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation |