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PKIG Polyclonal antibody

PKIG Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21371-1-AP

Print datasheet

Synonyms

PKI gamma, PKIG

验证数据展示

  • WB analysis of mouse heart using 21371-1-AP

    WB analysis of mouse heart using 21371-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21371-1-AP (PKIG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21371-1-AP

    IF Staining of HeLa using 21371-1-AP

    Immunofluorescent analysis of Hela cells, using PKIG antibody 21371-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21371-1-AP is PKIG Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PKIG fusion protein Ag15878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor gamma
Synonyms PKI gamma, PKIG
Calculated Molecular Weight 76 aa, 8 kDa
Observed Molecular Weight 5-8 kDa
GenBank Accession NumberBC104257
Gene Symbol PKIG
Gene ID (NCBI) 11142
RRIDAB_10858922
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2B9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKIG antibody 21371-1-APDownload protocol
IF protocol for PKIG antibody 21371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biol Chem

cAMP-Dependent Protein Kinase (PKA) Signaling is Impaired in the Diabetic Heart.

Authors - Lee B Bockus
View Article