验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17580-1-AP targets PKLR in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | PKLR fusion protein Ag11096 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pyruvate kinase, liver and RBC |
| Synonyms | PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK |
| Calculated Molecular Weight | 574 aa, 62 kDa, 585 aa, 63 kDa |
| Observed Molecular Weight | 58-62 kDa |
| GenBank Accession Number | BC025737 |
| Gene Symbol | PKLR |
| Gene ID (NCBI) | 5313 |
| RRID | AB_2268201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PKLR antibody 17580-1-AP | Download protocol |
| IHC protocol for PKLR antibody 17580-1-AP | Download protocol |
| IP protocol for PKLR antibody 17580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
