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验证数据展示

  • WB analysis of KEK293 cells using 25659-1-AP (same clone as 25659-1-PBS)

    WB analysis of KEK293 cells using 25659-1-AP (same clone as 25659-1-PBS)

    WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PKM2 antibody included
  • IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 25659-1-AP (same clone as 25659-1-PBS)

    IHC staining of human colon using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PKM2 antibody included
  • Featured Product
  • KD/KO验证

PKM Polyclonal antibody, PBS Only

PKM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25659-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.

产品信息

25659-1-PBS targets PKM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PKM fusion protein Ag20270 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PKM pyruvate kinase, muscle
别名
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC000481
基因名称 PKM
Gene ID (NCBI) 5315
RRIDAB_2880181
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14618
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.