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  • Featured Product
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PKM Polyclonal antibody

PKM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10078-2-AP

Print datasheet

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2

验证数据展示

  • IHC staining of human colon cancer using 10078-2-AP

    IHC staining of human colon cancer using 10078-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10078-2-AP (PKM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • WB analysis of HEK-293 cells using 10078-2-AP

    WB analysis of HEK-293 cells using 10078-2-AP

    WB result of PKM antibody (10078-2-AP, 1:1000) with si-Control and si-PKM transfected HEK293 cells.

  • WB analysis using 10078-2-AP

    WB analysis using 10078-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of multi-cells/tissue using 10078-2-AP

    WB analysis of multi-cells/tissue using 10078-2-AP

    WB result of 10078-2-AP (PKM antibody) with various lysates.

  • WB analysis of rat skeletal muscle using 10078-2-AP

    WB analysis of rat skeletal muscle using 10078-2-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10078-2-AP

    IF Staining of HeLa using 10078-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (10078-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 10078-2-AP

    FC experiment of HepG2 using 10078-2-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKM (10078-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, multi-cells/tissue, Raji cells, SH-SY5Y cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, NIH/3T3 cells, HepG2 cells, HEK-293 cells, rat skeletal muscle tissue, mouse brain tissue, mouse muscle tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10078-2-AP targets PKM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen PKM fusion protein Ag0110 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PKM pyruvate kinase, muscle
Synonyms PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC000481
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2283775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

实验方案

Product Specific Protocols
WB protocol for PKM antibody 10078-2-APDownload protocol
IHC protocol for PKM antibody 10078-2-APDownload protocol
IF protocol for PKM antibody 10078-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Authors - Sefan Asamitsu
View Article
pigWB

Br J Pharmacol

PMID: 30515768

Authors - Xiaoying Lian
View Article
humanWB

Cancer Sci

Identification of TPI1 As a potential therapeutic target in pancreatic cancer with dependency of TP53 mutation using multi-omics analysis

Authors - Tomoaki Toyoda
View Article
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article
humanWB,IP

J Cell Mol Med

METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination

Authors - Hao Wu
View Article
pigWB

Sci Rep

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Authors - Liangliang Fu
View Article