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验证数据展示

  • WB analysis using 84451-1-RR

    WB analysis using 84451-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84451-1-RR (PK-M2-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84451-1-RR

    IHC staining of human colon cancer using 84451-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84451-1-RR (PKM2-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human colon cancer using 84451-1-RR

    IHC staining of human colon cancer using 84451-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84451-1-RR (PKM2-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IF Staining of HeLa using 84451-1-RR

    IF Staining of HeLa using 84451-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (84451-1-RR, Clone: 241431E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84451-1-RR

    FC experiment of HeLa using 84451-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PKM2-specific Recombinant antibody (84451-1-RR, Clone:241431E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using 84451-1-RR

    FC experiment of HepG2 using 84451-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PKM2-specific Recombinant antibody (84451-1-RR, Clone:241431E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PKM2-specific Recombinant antibody

PKM2-specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84451-1-RR

产品说明书

CloneNo. 241431E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, A549 cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84451-1-RR targets PKM2-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PKM pyruvate kinase, muscle
别名 PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号NM_002654
基因名称 PKM
Gene ID (NCBI) 5315
RRIDAB_3671977
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP14618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

实验方案

Product Specific Protocols
WB protocol for PKM2-specific antibody 84451-1-RRDownload protocol
IHC protocol for PKM2-specific antibody 84451-1-RRDownload protocol
IF protocol for PKM2-specific antibody 84451-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols